Product Information
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Silica Magnetic Beads is Fe3O4 magnetic beads coated with a silicon dioxide (SiO2) layer. Since silica is able to bind to the nucleic acids, Silica Magnetic Beads serves as simple and efficient tool for plasmid DNA purification for transfection or sequencing applications, genomic DNA purification for research or clinical applications, RNA purification for qPCR analysis, or PCR product clean-up for downstream analysis.
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| Application | High adsorption & easy suspension |
| Characteristic of 1 mL | Suspension more than 2 min after mixing. | Core | Iron Oxide (Fe3O4) | Layer | Silica (SiO2) |
| Concentration | 50 mg/mL |
| Mean Diameter of Particles | 2.5 ~ 4.5 μm |
| Solution | ddH2O |
| Capacity | > 4 mg DNA/mL |
The application of Beads-Silica is relative characteristic of Algent Beads series that is all sedimentation particles. Difference brand will be different.
MBXE00006：The appearance of supernatant may black or dark brown color that is normal at a standstill due to high suspension of product characteristic, please feel free to use.
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• Binding Buffer, pH 8.0:
- 4 M Guanidinium thiocyanate
- 40 mM Tris
- 17.6 mM EDTA
• Wash Buffer, pH 8.0:
- 10 mM Tris-HCl buffer
- 1 mM EDTA
- 70% EtOH
• Elution Buffer, pH 8.0:
- 10 mM Tris-HCl
- 1 mM EDTA
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Preparation of the Beads for Use
1. Resuspend the Beads-Silica thoroughly by pipetting or vortexing the vial.
2. Transfer an adequate amount of Beads-Silica into a clean tube.
3. Place the tube on the magnetic stand for 30-60 seconds to immobilize the beads at the tube wall.
4. Discard the supernatant by aspiration with a pipette.
5. Remove the tube from the magnetic stand.
6. Add 100 μL Elution Buffer (or ddH₂O) and resuspend the beads by pipetting or vortexing.
7. Place the tube on the magnetic stand for 30-60 seconds to immobilize the beads at the tube wall.
8. Discard the supernatant, then remove the tube from the magnetic stand.
9. Repeat steps 6-8 twice.
10. The beads are now ready for purification of nucleic acid.
Purification of Nucleic Acid
11. Mix 10 μL sample and 90 μL Binding Buffer with the beads thoroughly by pipetting.
12. Incubate with tilt rotation for 2 minutes at room temperature.
13. Place the tube on the magnetic stand for 30-60 seconds to immobilize the beads at the tube wall.
14. Discard (or collect) the supernatant as unbound substances by aspiration with a pipette, then remove the tube from the magnetic stand.
15. Add 100 μL Wash Buffer and resuspend the beads by pipetting.
16. Place the tube on the magnetic stand for 30-60 seconds to immobilize the beads at the tube wall.
17. Discard (or collect) the supernatant as unbound substances, then remove the tube from the magnetic stand.
18. Repeat steps 15-17 twice.
19. Air-dry with shaking for 5-20 minutes.
20. Proceed to elution of nucleic acid.
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