WB 

Western Blot. Western blotting is a technique used to detect specific proteins within a sample. It involves separating proteins by gel electrophoresis, transferring them to a membrane, and identifying the target proteins using specific antibodies.

IHC

Immunohistochemistry. Immunohistochemistry allows researchers to visualize specific proteins within tissue sections. By using labeled antibodies, it is possible to localize proteins in the context of tissue architecture, providing insight into protein expression and localization.

IP

Immunoprecipitation. Immunoprecipitation is used to isolate a specific antigen from a complex mixture using an antibody. This technique is essential for studying protein-protein interactions, post-translational modifications, and protein complex formation.

ChIP

Chromatin Immunoprecipitatio. ChIP is a method used to investigate protein-DNA interactions. By cross-linking proteins to DNA, followed by immunoprecipitation with specific antibodies, ChIP allows for the identification of DNA regions bound by proteins such as transcription factors.

FC

Flow Cytometry. Flow cytometry is a powerful tool for analyzing the physical and chemical characteristics of cells or particles in suspension. It allows for the simultaneous multi-parameter analysis of thousands of cells, making it invaluable for immune profiling and cell sorting.

ELISA

Enzyme-Linked Immunosorbent Assay. ELISA is a plate-based assay technique used to detect and quantify soluble substances such as proteins, peptides, or antibodies. This highly sensitive method is widely used in diagnostics and research to measure concentrations of target molecules.

DB

Dot Blot. Dot blot is a simple method to detect specific biomolecules. A small sample is applied directly onto a membrane, and target molecules are identified using antibodies, similar to Western blot, but without the need for gel electrophoresis.

Conjugation

Conjugation. Conjugation refers to the process of linking biomolecules such as antibodies to labels like enzymes, fluorophores, or biotin. This process enhances detection in various assays, enabling more accurate and specific targeting of molecules.

Purification

Purification. Protein purification involves isolating proteins from complex mixtures to study their structure and function. Techniques like affinity chromatography or ion-exchange chromatography are commonly used to achieve high purity and yield.