At Algent Bio, we understand that reliable antibodies are the cornerstone of experimental success. Antibody validation involves generating robust experimental evidence and reporting to demonstrate that a specific antibody performs as intended for its given application (Weller, 2018). The issue of reproducibility in scientific research has led to the development of stringent validation measures, including the formation of the International Working Group for Antibody Validation (IWGAV), which has outlined five key recommendations for antibody validation (Uhlen et al., 2016). These include genetic validation, positive interaction strategies, independent antibody verification, tagged protein expression, and immunocapture coupled with mass spectrometry.

To ensure that Algent Bio's antibodies deliver the most reliable results in your experiments, we employ a rigorous validation process that aligns with these international standards. With over years of expertise in antibody production, Algent Bio employs a tailored combination of validation techniques for each antibody, including: