Product Information
Downloads & Supports
Beads-Protein A is designed as a rapid and simple tool or immunoprecipitation, purification/ depletion assays, and other magnetic separation applications. Antibody can easily bind to the Beads due to its’ high affinity with protein A. Via the antibody specific binding ability, the target protein along with Beads-Protein A could be temporarily immobilized at tube wall, so the other parts in the supernatant can be removed easily and efficiently under magnetic attraction.
Binding characteristics
The binding strength of Beads-Protein A to different immunoglobulins is listed as below:
Table 1. Binding strength of Beads-Protein A
| Species | Binding strength |
|---|---|
| Human IgG (normal) | ＋＋＋＋ |
| Mouse IgG1 | ＋ |
| Rat IgG1 | － |
| Goat IgG | ＋/－ |
| Rabbit IgG | ＋＋＋＋ |
| Chicken IgG | － |
Specifications
| Core | Iron Oxide (Fe3O4) |
| Layer | Dextran |
| Functional group | Protein A |
| Mean Diameter of Particles | ~1 μm |
| Storage Buffer | pH 7.4 PBS, 0.02% Tween 20 and 0.09% sodium azide. |
| Storage temp. | 2 ~ 8 ℃ |
| Capacity | 260 ug human IgG/1 ml |
Material supplied
-
Beads-Protein A provides Fe3O4 beads coated with dextran of an average ~1 μm in diameter. Protein A, about 42 kDa, is coupled covalently to dextran. Beads are supplied in phosphate buffered saline, pH 7.4, containing 0.02% Tween 20 and 0.09% sodium azide.
Additional material required
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• Washing buffer: PBS buffer (pH 7.4) with 0.02% Tween 20
• Elution buffer if necessary
• Neutralization buffer if necessary
• Desired antibody
• Magntic stand: Magdorf is suggested for the best performance
• Tilt rotation device or vortexer
• Eppendorf tubes & pipett