The PI3-kinase p85-alpha/gamma Colorimetric Cell-Based ELISA Kit provides a lysate-free, convenient, and high-throughput approach for sensitive detection of PI3-kinasep85-alpha/gamma protein expression profile in cells. The kit can be used for measuring the relative amounts of PI3-kinasep85-alpha/gamma in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on PI3-kinasep85-alpha/gamma.