Phospho-IKK-alpha (Ser176) /IKK-beta (Ser177) Fluorometric Cell-Based ELISA Kit
Phospho-IKK-alpha (Ser176) /IKK-beta (Ser177) Fluorometric Cell-Based ELISA Kit

Phospho-IKK-alpha (Ser176) /IKK-beta (Ser177) Fluorometric Cell-Based ELISA Kit

The Phospho-IKK-alpha (Ser176) /IKK-beta (Ser177) Fluorometric Cell-Based ELISA Kit provides a lysate-free, convenient, and high-throughput approach for monitoring Phospho-IKK-alpha (Ser176) /IKK-beta (Ser177) protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated Phospho-IKK-alpha (Ser176) /IKK-beta (Ser177) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals, or activators have on CAV1 phosphorylation.

Reactivity
  • Human
  • Mouse
  • Rat
Application
  • ELISA
  • Cell-Based ELISA
Sample Type
    Researchers plate their cell line of choice
Catalog Number
    ECBC2125
$460.00
Unit
    2 Plates: TC 96-Well Plates
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Product Information

Catalog Number
    ECBC2125

Downloads & Supports

Target
    Phospho-IKK-alpha (Ser176) /IKK-beta (Ser177)
Sample Type
    Researchers plate their cell line of choice
Detection Method
    Fluorometric
Storage
    4°C/6 Months
Assay Type
  • Cell-Based ELISA
Reactivity
  • Human
  • Mouse
  • Rat
Assay Range
    At least 5000 cells/well
Assay Time
    4.5 hours
Application
  • ELISA
  • Cell-Based ELISA
Synonyms
    CHUK; Conserved helix-loop-helix ubiquitous kinase; I kappa-B kinase alpha; I-kappa-B kinase 1; IkappaB kinase; IkBKA; IKK-A; IKK-alpha; IKK1; IKKA; Inhibitor of nuclear factor kappa-B kinase alpha subunit; kinase IKK-alpha; NFKBIKA; Nuclear factor NFkappaB inhibitor kinase alpha; I-kappa-B kinase 2; I-kappa-B-kinase beta; IkBKB; IKK-B; IKK-beta; IKK2; IKKB; kinase IKK-beta; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase beta
Gene ID
    1147/3551
Uniprot ID
    O15111/O14920
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Product Image

Western blot analysis of extracts from NIH-3T3 cells treated with TNF 20ng/ml 30', using IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Antibody.