The Phospho-HDAC5 (Ser259) Fluorometric Cell-Based ELISA Kit provides a lysate-free, convenient, and high-throughput approach for monitoring Phospho-HDAC5 (Ser259) protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated Phospho-HDAC5 (Ser259) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals, or activators have on CAV1 phosphorylation.