The p70 S6 Kinase Colorimetric Cell-Based ELISA Kit provides a lysate-free, convenient, and high-throughput approach for sensitive detection of p70S6Kinase protein expression profile in cells. The kit can be used for measuring the relative amounts of p70S6Kinase in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on p70S6Kinase.