Product Information
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NTA-Ni Magnetic Beads is designed for rapid purification of 6xHis-tagged proteins. NTA-Ni Magnetic Beads has nitrilo- triacetic acid (NTA) groups with charged nickel covalently bind to surface dextran of Beads. Due to the high affinity, NTA-Ni Magnetic Beads can be used for capturing 6xHis-tagged proteins. Bound 6xHis-tagged proteins can be temporarily immobilized under magnetic attraction, so the other parts in supernatant can be removed easily and efficiently. Bound proteins can be directly used in downstream applications or be eluted off the beads. The capacity of purified 6xHis-tagged proteins (~35kDa) captured by NTA-Ni Magnetic Beads is approximately 5 mg/mL.
Binding characteristics
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Specifications
| Core | Iron Oxide (Fe3O4) |
| Layer | Dextran |
| Surface protein | NTA-Ni |
| Mean Diameter of Particles | ~1 μm |
| Storage Buffer | 20% ethanol |
| Storage temp. | 2 ~ 8 ℃ |
| Capacity | 5 mg/mL |
Material supplied
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Beads-NTA-Ni contains coated magnetic beads of an average ~1 μm in diameter. The beads are suspended in 20% ethanol reagent.
Additional material required
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• Beads-NTA-Ni Binding/Wash Buffer
- 50 mM sodium phosphate, pH 7.4
- 300 mM NaCl
- 0.02 % Tween 20
• Magnetic stand: Magdorf (MDF-08) for the best performance
• Tilt rotation device
• Beads-NTA-Ni Elution Buffer
- 50 mM sodium phosphate, pH 7.0
- 300 mM NaCl
- 500 mM Imidazole
- 0.1 % Tween 20
• Eppendorf tubes & pipettes