The NKX6.3 Colorimetric Cell-Based ELISA provides a lysate-free, convenient, and high-throughput approach for sensitive detection of NKX6.3 protein expression profile in cells. The kit can be used for measuring the relative amounts of NKX6.3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on NKX6.3.