Product Information
Downloads & Supports
Magnetic Separator can operate 8 samples at the same time.
With strong magnetic force, it can quickly collect micron size magnetic beads and the magnetic beads are collected in a small region.
Specifications
| Size | 120mm (length) X 52mm (width) X 55mm (height) |
| Number of operating wells | 8 |
Troubleshooting
| Troubles | Solutions |
| Immunoglobin binding is low. | 1. Ensure that the beads are thoroughly suspended before use. 2. Thoroughly and continuously mix the beads and sample using either a tilt rotation device or a vortexer. 3. Refer to Table 1 to match Protein A’s binding preferences with various immunoglobulins. 4. Optimize incubation time and temperature based on the sample volume and the antibody’s affinity for the target antigen. |
| Non-specific and background binding is high. | 1. Reduce the amount of beads used per test according to the binding capacity mentioned in "Binding Characteristics". 2. After incubating the beads-antibody complex with the antigen (Step 19), increase the number of wash steps (Step 20) to five. 3. Increase the concentration of Tween 20 to 0.1% in the washing buffer before eluting the sample. 4. Ensure that the washing buffer is completely removed. |
| Beads do not collect on the magnet. | 1. Make sure the tube is in direct contact with the magnet. 2. Use the Magstand for best performance. |