Loading Control Mouse Monoclonal Antibody Kit
Product Information
Downloads & Supports
Target
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ACTB/TUBB/GAPDH
Host
Storage
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Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Clonality
Clone Number
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Isotype
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Beta actin: IgG2b; Beta Tubulin: IgG1; GAPDH: IgM
Reactivity
Application
Application Details
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WB: 1/5000-1/10000
Immunogen
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Molecular Weight
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Beta actin: 42 kDa; Beta Tubulin: 55 kDa; GAPDH: 36 kDa
Formulation
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Beta actin: Purified mouse monoclonal in PB(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.Beta Tubulin: Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide 0.1mg/mlBSA and 50% glycerol.GAPDH: Ascites containing with 0.03% Proclin300 and 50% glycerol.
Form
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Liquid
Gene ID
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60/203068/2597
Uniprot ID
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P60709/P07437/P04406
Background
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This kit is consist of three different but reliable antibodies which are specific to the most popular three loading control targets: GAPDH, β-Actin and β-Tubulin, also with three individual package.
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells(Swiss-Prot Acc.P60709).
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain (Swiss-Prot Acc.P07437).
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis, gene expression, and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer diseases (Swiss-Prot Acc.P04406).
Research Area
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Synonyms
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ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta; Beta 4 tubulin; TBB5; TUBB2; TUBB2A; tubulin beta 2A; beta tubulin; tubulin beta chain; G3PD; GAPD; GAPDH; HEL-S-162eP