The LIMK-2 (Phospho-Thr505) Colorimetric Cell-Based ELISA Kit provides a lysate-free, convenient, and high-throughput approach for sensitive detection of LIMK-2 (Phospho-Thr505) protein expression profile in cells. The kit can be used for measuring the relative amounts of LIMK-2 (Phospho-Thr505) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on LIMK-2 (Phospho-Thr505).