The LIMK-1/2 (Phospho-Thr508/505) Colorimetric Cell-Based ELISA Kit provides a lysate-free, convenient, and high-throughput approach for sensitive detection of LIMK-1/2 (Phospho-Thr508/505) protein expression profile in cells. The kit can be used for measuring the relative amounts of LIMK-1/2 (Phospho-Thr508/505) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on LIMK-1/2 (Phospho-Thr508/505).