Leptin Rat Recombinant Protein, PEG

Algent bio Protein
Algent bio Protein

Leptin Rat Recombinant Protein, PEG

Mono-Pegylated Leptin Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and an additional Ala at N-terminus having a molecular mass of 35.6 kDa (with 20 kDa PEG) as determined by mass spectometry. However due to enlarged hydrodymanic volume it runs on the SDS-PAGE as 48 kDa protein and in gel-filtration on Superdex 200 as over 100 kDa protein. Its half-life in circulation after SC injection was over 20 hours. Rat Leptin was purified by proprietary chromatographic techniques according to Salomon et al (2006) Protein Expression and Purification 47, 128–136 and then pegylated.

Catalog Number
    PRXD02603
$350.00

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United States

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Catalog Number
    PRXD02603
Source
    Escherichia Coli.
Form
    Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
    The protein was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3.
Stability
    Lyophilized Rat Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Rat Leptin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Uniprot ID
    P50596
Purity
    Greater than 99.0% as determined by:(a) Analysis by Gel-Filtration.(b) Analysis by SDS-PAGE.
Synonyms
    OB Protein, Obesity Protein, OBS, Obesity factor.
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Usage: Algentbio products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.