His Tag (2A2) Mouse Monoclonal Antibody

His Tag (2A2) Mouse Monoclonal Antibody
His Tag (2A2) Mouse Monoclonal Antibody
His Tag (2A2) Mouse Monoclonal Antibody
His Tag (2A2) Mouse Monoclonal Antibody
His Tag (2A2) Mouse Monoclonal Antibody
His Tag (2A2) Mouse Monoclonal Antibody

His Tag (2A2) Mouse Monoclonal Antibody

Mouse IgG1 Monoclonal for - detection, Test with WB, ICC/IF, IP in Species-independent

Reactivity
  • Species-independent
Application
  • WB
  • ICC
  • IF
  • IP
Host
  • Mouse
Catalog Number
    TGXB00036
$220.00

Shipping To:
United States

Product Information

Downloads & Supports

Target
    -
Host
  • Mouse
Storage
    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
  • Monoclonal
Clone Number
    2A2-4H2-10D8
Isotype
    IgG1
Reactivity
  • Species-independent
Application
  • WB
  • ICC
  • IF
  • IP
Application Details
    WB: 1/1000-1/10000 IF: 1/100-1/500 IP: 1/200
Immunogen
    Synthetic peptide: HHHHHH conjugated to KLH.
Molecular Weight
    Refer to figures
Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Form
    Liquid
Gene ID
    -
Uniprot ID
    -
Background
    A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
Research Area
    Tags & Cell Markers
Synonyms
    6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag
MSDSProtocolsRequest COAAlgent Product GuaranteeNeed support?

Product Image

2ug His fusion protein+ Primary antibody dilution at 1) 1:5000. 2) 1:10000
Immunofluorescence analysis of His Tag (2A2) in 293 cells transfected with a Histag protein using His Tag (2A2) antibody(red),and DAPI (blue).
Immunoprecipitation analysis of Flag Tag (7E1) in 1、untransfected 293 cell lysates2、transfected 293 cell lysates with Histag fusion protein3、IP (untransfected 293+antiHis mAb+ Protein G agarose)4、IP (transfected 293+ normal mouse IgG+Protein G agarose)5、IP (transfected 293+antiHis mAb+ Protein G agarose)