His Tag (1H3) Mouse Monoclonal Antibody

His Tag (1H3) Mouse Monoclonal Antibody
His Tag (1H3) Mouse Monoclonal Antibody

His Tag (1H3) Mouse Monoclonal Antibody

Mouse IgG1 Monoclonal for - detection, Test with WB in Species-independent

Reactivity
  • Species-independent
Application
  • WB
Host
  • Mouse
Catalog Number
    TGXB00050
$220.00

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United States

Product Information

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Target
    -
Host
  • Mouse
Storage
    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
  • Monoclonal
Clone Number
    1H3-A2
Isotype
    IgG1
Reactivity
  • Species-independent
Application
  • WB
Application Details
    WB: 1/1000-1/10000
Immunogen
    Synthetic peptide corresponding to fragment (HHHHHH) His Tag
Molecular Weight
    Refer to figures
Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Form
    Liquid
Gene ID
    -
Uniprot ID
    -
Background
    A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
Research Area
    Tags & Cell Markers
Synonyms
    6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag
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Product Image

Western blot analysis of Histag in various bacterial lysates, which containing Histagged proteins (MW: 30 kDa, 45 kDa, 72 kDa, 44 kDa and 40 kDa respectively) using antihis antibody.