The Hexokinase-3 Colorimetric Cell-Based ELISA provides a lysate-free, convenient, and high-throughput approach for sensitive detection of Hexokinase-3 protein expression profile in cells. The kit can be used for measuring the relative amounts of Hexokinase-3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on Hexokinase-3.