The Glucokinase Regulator Colorimetric Cell-Based ELISA provides a lysate-free, convenient, and high-throughput approach for sensitive detection of GlucokinaseRegulator protein expression profile in cells. The kit can be used for measuring the relative amounts of GlucokinaseRegulator in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on GlucokinaseRegulator.