GAPDH Rabbit Monoclonal Antibody
Product Information
Downloads & Supports
Target
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GAPDH
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
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R08-4C3
Isotype
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IgG
Reactivity
Application
Application Details
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WB: 1/5000-1/10000 IHC: 1/50-1/200 IF: 1/100-1/200
Immunogen
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Recombinant protein of human GAPDH
Molecular Weight
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Calculated MW: 36 kDa; Observed MW: 36 kDa
Formulation
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50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Form
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Liquid
Gene ID
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2597
Uniprot ID
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P04406
Background
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Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.
Research Area
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Neuroscience
Synonyms
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G3PD; GAPD; HEL-S-162eP