Flag Tag (7E1) Mouse Monoclonal Antibody

Flag Tag (7E1) Mouse Monoclonal Antibody
Flag Tag (7E1) Mouse Monoclonal Antibody
Flag Tag (7E1) Mouse Monoclonal Antibody
Flag Tag (7E1) Mouse Monoclonal Antibody
Flag Tag (7E1) Mouse Monoclonal Antibody
Flag Tag (7E1) Mouse Monoclonal Antibody

Flag Tag (7E1) Mouse Monoclonal Antibody

Mouse IgG1 Monoclonal for - detection, Test with WB, ICC/IF, IP in Species-independent

Reactivity
  • Species-independent
Application
  • WB
  • ICC
  • IF
  • IP
Host
  • Mouse
Catalog Number
    TGXB00048
$220.00

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United States

Product Information

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Target
    -
Host
  • Mouse
Storage
    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
  • Monoclonal
Clone Number
    7E1-10D2-3H1
Isotype
    IgG1
Reactivity
  • Species-independent
Application
  • WB
  • ICC
  • IF
  • IP
Application Details
    WB: 1/1000-1/10000 IF: 1/100-1/500 IP: 1/20
Immunogen
    Synthetic Peptide of Flag-Tag
Molecular Weight
    Refer to figures
Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Form
    Liquid
Gene ID
    -
Uniprot ID
    -
Background
    Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.
Research Area
    Tags & Cell Markers
Synonyms
    DYKDDDDK Tag; Flag; FLAG
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Product Image

Immunofluorescence analysis of Flag Tag (7E1)) in 293 cells transfected with a Flagtag protein using Flag Tag (7E1) antibody(red),and DAPI (blue).
Immunoprecipitation analysis of Flag Tag (7E1) in 1) untransfected 293 cell lysates 2) transfected 293 cell lysates with Flagtag fusion protein 3) IP (transfected 293+ normal mouse IgG+Protein G agarose) 4) IP (transfected 293+anti Flag mA+Protein G agarose).
Western blot analysis of Flag Tag (7E1) in CHO-K1 cells (C) or CHO-K1 cells transfected with different FLAGfusion proteins (A, B), using FlagTag antibody.