Flag Tag (10E1) Mouse Monoclonal Antibody
Product Information
Downloads & Supports
Target
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Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
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10E1-1D8-5F5
Isotype
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IgG2b
Reactivity
Application
Application Details
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WB: 1/1000-1/10000 IP: 1/500
Immunogen
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Monoclonal antibodies are produced by immunizing mouse with synthetic peptide (DYKDDDDK), coupled to KLH.
Molecular Weight
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Refer to figures
Formulation
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Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Form
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Liquid
Gene ID
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Uniprot ID
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Background
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Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.
Research Area
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Tags & Cell Markers
Synonyms
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DYKDDDDK Tag; Flag; FLAG