The Ezrin (Phospho-Thr566) Colorimetric Cell-Based ELISA Kit provides a lysate-free, convenient, and high-throughput approach for sensitive detection of Ezrin (Phospho-Thr566) protein expression profile in cells. The kit can be used for measuring the relative amounts of Ezrin (Phospho-Thr566) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on Ezrin (Phospho-Thr566).