The Cytochrome P450 39A1 Colorimetric Cell-Based ELISA provides a lysate-free, convenient, and high-throughput approach for sensitive detection of CytochromeP45039A1 protein expression profile in cells. The kit can be used for measuring the relative amounts of CytochromeP45039A1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on CytochromeP45039A1.