beta Actin (8F10) Mouse Monoclonal Antibody (HRP)
Product Information
Downloads & Supports
Target
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ACTB
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
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8F10-G10
Isotype
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IgG2b
Reactivity
Application
Application Details
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WB: 1/5000-1/10000
Immunogen
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Purified recombinant human beta-actin protein fragments expressed in E.coli.
Molecular Weight
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Calculated MW: 42 kDa; Observed MW: 42 kDa
Formulation
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Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Form
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Liquid
Gene ID
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60
Uniprot ID
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P60709
Background
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beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.
Research Area
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Cell Biology
Synonyms
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ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta