Anti-YTHDF1 Mouse Monoclonal Antibody
Product Information
Downloads & Supports
Target
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YTHDF1
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
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5C3-Y4-X7
Isotype
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IgG1/Kappa
Reactivity
Application
Application Details
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WB：1/500-1/1000
Immunogen
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Peptide
Molecular Weight
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Calculated MW: 61 kDa; Observed MW:68-70 kDa
Formulation
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Liquid in PBS, Glycerol and BSA
Form
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Liquid
Gene ID
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54915
Uniprot ID
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Q9BYJ9
Background
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Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and regulates their stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing. Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT complex. The YTHDF paralogs (YTHDF1, YTHDF2 and YTHDF3) shares m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation. Required to facilitate learning and memory formation in the hippocampus by binding to m6A-containing neuronal mRNAs. Acts as a regulator of axon guidance by binding to m6A-containing ROBO3 transcripts. Acts as a negative regulator of antigen cross-presentation in myeloid dendritic cells. In the context of tumorigenesis, negative regulation of antigen cross-presentation limits the anti-tumor response by reducing efficiency of tumor-antigen cross-presentation. Promotes formation of phase-separated membraneless compartments, such as P-bodies or stress granules, by undergoing liquid-liquid phase separation upon binding to mRNAs containing multiple m6A-modified residues: polymethylated mRNAs act as a multivalent scaffold for the binding of YTHDF proteins, juxtaposing their disordered regions and thereby leading to phase separation.
Research Area
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Cell Biology
Synonyms
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DF1, DACA-1, C20orf21