Anti-TriMethyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody

Anti-TriMethyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody
Anti-TriMethyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody
Anti-TriMethyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody
Anti-TriMethyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody
Anti-TriMethyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody
Anti-TriMethyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody
Anti-TriMethyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody
Anti-TriMethyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody
Anti-TriMethyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody
Anti-TriMethyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody
Anti-TriMethyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody
Anti-TriMethyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody

Anti-TriMethyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody

Rabbit IgG Polyclonal for H3-4 detection, Test with WB, IHC-P, ICC/IF, ChIP in Human, Mouse, Rat

Reactivity
  • Human
  • Mouse
  • Rat
Application
  • WB
  • IHC-P
  • ICC
  • IF
  • ChIP
Host
  • Rabbit
Catalog Number
    ABXB06636
$490.00

Shipping To:
United States

Product Information

Downloads & Supports

Target
    H3-4
Host
  • Rabbit
Storage
    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
  • Polyclonal
Clone Number
    -
Isotype
    IgG
Reactivity
  • Human
  • Mouse
  • Rat
Application
  • WB
  • IHC-P
  • ICC
  • IF
  • ChIP
Application Details
    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ChIP: 1:20 - 1:100
Immunogen
    A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
Molecular Weight
    Calculated MW: 16 kDa; Observed MW: 16 kDa
Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Form
    Liquid
Gene ID
    8290
Uniprot ID
    Q16695
Background
    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Research Area
    Epigenetics and Nuclear Signaling
Synonyms
    H3K9me3; H3 histone; HIST1H3A; Histone cluster 1; H3a
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Product Image

Western blot analysis of TriMethyl-Histone H3 (Lys9) in various cell lines lysates using TriMethyl-Histone H3K9.
TriMethyl-Histone H3K9 Dotblot analysis of all sorts of methylation peptides using TriMethyl-Histone H3K9.
Immunohistochemistry analysis of paraffin-embedded rat testis using TriMethyl-Histone H3K9. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Immunohistochemistry analysis of paraffin-embedded mouse testis using TriMethyl-Histone H3K9.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Immunofluorescence analysis of TriMethyl-Histone H3 (Lys9) in 293T using TriMethyl-Histone H3K9,and DAPI (blue).
Chromatin Immunoprecipitation TriMethyl-Histone H3K9 pAb Chromatin immunoprecipitation analysis of extracts of 293T cells, using TriMethyl-Histone H3K9 and rabbit IgG. P1 and P2 were located on EBAG9 gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the rat ios of the immunoprecipitated DNA to the input.