Anti-Smad3 (4H4) Mouse Monoclonal Antibody

Anti-Smad3 (4H4) Mouse Monoclonal Antibody
Anti-Smad3 (4H4) Mouse Monoclonal Antibody
Anti-Smad3 (4H4) Mouse Monoclonal Antibody
Anti-Smad3 (4H4) Mouse Monoclonal Antibody
Anti-Smad3 (4H4) Mouse Monoclonal Antibody
Anti-Smad3 (4H4) Mouse Monoclonal Antibody

Anti-Smad3 (4H4) Mouse Monoclonal Antibody

Mouse IgG1 Monoclonal for SMAD3 detection, Test with WB, IHC-P in Human, Rat, Mouse

Reactivity
  • Human
  • Rat
  • Mouse
Application
  • WB
  • IHC-P
Host
  • Mouse
Catalog Number
    ABXB05547
$370.00

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United States

Product Information

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Target
    SMAD3
Host
  • Mouse
Storage
    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
  • Monoclonal
Clone Number
    4H4-10A10-7B4
Isotype
    IgG1
Reactivity
  • Human
  • Rat
  • Mouse
Application
  • WB
  • IHC-P
Application Details
    WB: 1/500-1/1000 IHC: 1/50-1/100
Immunogen
    Purified recombinant protein expressed in E.coli.
Molecular Weight
    Calculated MW: 48 kDa; Observed MW: 48 kDa
Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Form
    Liquid
Gene ID
    4088
Uniprot ID
    P84022
Background
    Smad3 transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.
Research Area
    Signal Transduction
Synonyms
    SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
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Western blot analysis of Smad3 (4H4)in 293T, Jurkat, rat Heart, mouse Heart lysates using Smad3.
Immunohistochemistry analysis of paraffin-embedded Human Liver Tissue using Smad3.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Immunohistochemistry analysis of paraffin-embedded Human Colon Carcinoma using Smad3. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.