Anti-SARS CoV/SARS CoV 2 Spike Human mAb
Product Information
Downloads & Supports
Target
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S
Host
Storage
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Store at 4°C for at least 6 months. Avoid freeze/thaw cycles
Clonality
Clone Number
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-
Isotype
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IgG
Reactivity
Application
Application Details
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WB: 1/500-1/1000
Immunogen
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A synthesized peptide derived from SARS CoV/SARS CoV 2 Spike
Molecular Weight
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Calculated MW: 141 kDa; Observed MW: 141 kDa
Formulation
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0.01M PBS, pH 7.2
Form
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Liquid
Gene ID
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43740568
Uniprot ID
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P0DTC2
Background
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Spike protein S1: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 and CLEC4M/DC-SIGNR receptors and internalization of the virus into the endosomes of the host cell induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.UniRule annotation2 Publications Spike protein S2: mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.UniRule annotation Spike protein S2': Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.UniRule annotation1 Publication
Research Area
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Infectious Diseases
Synonyms
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SARS CoV 2 Spike; Peplomer protein; E2