Anti-Ras(Q61H Mutant) Mouse Monoclonal Antibody

Anti-Ras(Q61H Mutant) Mouse Monoclonal Antibody
Anti-Ras(Q61H Mutant) Mouse Monoclonal Antibody
Anti-Ras(Q61H Mutant) Mouse Monoclonal Antibody
Anti-Ras(Q61H Mutant) Mouse Monoclonal Antibody

Anti-Ras(Q61H Mutant) Mouse Monoclonal Antibody

Mouse IgG Monoclonal for RAS detection, Test with WB, IHC-P, ICC/IF, ELISA in Vertebrates

Reactivity
  • Vertebrates
Application
  • WB
  • IHC-P
  • ICC
  • IF
  • ELISA
Host
  • Mouse
Catalog Number
    ABXB07384
$680.00

Shipping To:
United States

Product Information

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Target
    RAS
Host
  • Mouse
Storage
    Store at -20°C. Avoid repeated freezing and thawing
Clonality
  • Monoclonal
Clone Number
    7R4-B6-A3
Isotype
    IgG
Reactivity
  • Vertebrates
Application
  • WB
  • IHC-P
  • ICC
  • IF
  • ELISA
Application Details
    ELISA 1/100-1/5000 WB 1/100-1/2000 ICC/IF 1/50-1/100 IHC-P 1/50-1/100
Immunogen
    A synthetic peptide from the internal region of Ras which includes the mutation of Q61H, human origin
Formulation
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Form
    Liquid
Uniprot ID
    ­
Background
    Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras Q61H mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.
Research Area
    Cell Biology
Synonyms
    RAS
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Product Image

Western blot analysis of recombinant Ras proteins. Equal amount of purified Ras Q61H (lane 1), Ras Q61L (lane 2), Ras Q61R (lane 3), kRas WT (lane 4), hRas WT (lane 5), then were loaded and blotted with anti-Ras(Q61H) mouse monoclonal. 
Immunofluorescence of cells expressing Ras proteins with Anti-Ras(Q61H). HEK293T cells were transfected with pCDNA3-GFP-Ras(Q61H) plasmid, pCDNA3-GFP-Ras (WT) plasmid, pCDNA3-GFP-Ras (Q61L) plasmid, pCDNA3-GFP-Ras (Q61R) plasmid or pCDNA3-GFP-Ras (Q61K) plasmid, then fixed and stained with Anti-Ras(Q61H) monoclonal.