Anti-Ras(G12S Mutant) Mouse Monoclonal Antibody

Anti-Ras(G12S Mutant) Mouse Monoclonal Antibody
Anti-Ras(G12S Mutant) Mouse Monoclonal Antibody
Anti-Ras(G12S Mutant) Mouse Monoclonal Antibody
Anti-Ras(G12S Mutant) Mouse Monoclonal Antibody
Anti-Ras(G12S Mutant) Mouse Monoclonal Antibody
Anti-Ras(G12S Mutant) Mouse Monoclonal Antibody

Anti-Ras(G12S Mutant) Mouse Monoclonal Antibody

Mouse IgG Monoclonal for H-Ras, K-Ras, N-Ras detection, Test with WB, IHC-P, ELISA in Vertebrates

Reactivity
  • Vertebrates
Application
  • WB
  • IHC-P
  • ELISA
Host
  • Mouse
Catalog Number
    ABXB07381
$680.00

Shipping To:
United States

Product Information

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Target
    H-Ras, K-Ras, N-Ras
Host
  • Mouse
Storage
    Store at -20°C. Avoid repeated freezing and thawing
Clonality
  • Monoclonal
Clone Number
    8I2-I9-W4
Isotype
    IgG
Reactivity
  • Vertebrates
Application
  • WB
  • IHC-P
  • ELISA
Application Details
    ELISA: 1/1000-1/5000 WB: 1/500-1/1000 IHC-P: 1/50-1/100
Immunogen
    A synthetic peptide from the internal region of RAS which includes the mutation of G12S, human origin.
Formulation
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Form
    Liquid
Uniprot ID
    ­
Background
    Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras G12S mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.
Research Area
    Cell Biology
Synonyms
    H-Ras, K-Ras, N-Ras
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Product Image

Western blot analysis of recombinant RAS(G12S) and wild type proteins. Purified His-tagged RAS(G12S) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-RAS(G12S) monoclonal.
Immunofluorescence of cells expressing RAS proteins with Anti-RAS(G12S). HEK293T cells were transfected with pCDNA3-GFP-Ras(G12S) plasmid, pCDNA3-GFP-Ras(WT)plasmid,pCDNA3-GFP-Ras(G12S) plasmid, pCDNA3-GFP-Ras(G12V)plasmid,pCDNA3-GFP-Ras(G12C)plasmid, pCDNA3-GFP-Ras(G12R)plasmid or pCDNA3-GFP-Ras(G12A)plasmid, then fixed and stained with Anti-RAS(G12S) monoclonal.