Anti-Ras(A146T Mutant) Mouse Monoclonal Antibody

Anti-Ras(A146T Mutant) Mouse Monoclonal Antibody
Anti-Ras(A146T Mutant) Mouse Monoclonal Antibody
Anti-Ras(A146T Mutant) Mouse Monoclonal Antibody
Anti-Ras(A146T Mutant) Mouse Monoclonal Antibody

Anti-Ras(A146T Mutant) Mouse Monoclonal Antibody

Mouse IgG Monoclonal for H-Ras, K-Ras, N-Ras detection, Test with WB, IHC-P, ELISA in Vertebrates

Reactivity
  • Vertebrates
Application
  • WB
  • IHC-P
  • ELISA
Host
  • Mouse
Catalog Number
    ABXB07385
$680.00

Shipping To:
United States

Product Information

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Target
    H-Ras, K-Ras, N-Ras
Host
  • Mouse
Storage
    Store at -20°C. Avoid repeated freezing and thawing
Clonality
  • Monoclonal
Clone Number
    8I3-Z3-X8
Isotype
    IgG
Reactivity
  • Vertebrates
Application
  • WB
  • IHC-P
  • ELISA
Application Details
    ELISA: 1/1000-1/5000 WB: 1/500-1/1000 IHC-P: 1/50-1/100
Immunogen
    A synthetic peptide from the internal region of Ras which includes the mutation of A146T, human origin.
Formulation
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Form
    Liquid
Uniprot ID
    ­
Background
    The A146T mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras A146T mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.
Research Area
    Cell Biology
Synonyms
    H-Ras, K-Ras, N-Ras
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Product Image

Western blot analysis of recombinant HRAS(A146T) and wild type proteins. Purified His-tagged RAS(A146T) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-RAS(A146T) monoclonal.
Immunofluorescence of cells expressing HRas proteins with anti-HRAS(A146T). HEK293T cells were transfected with pCDNA3-GFP-HRas (WT) plasmid (left column) or pCDNA3-GFP-HRAS(A146T) plasmid (right column), then fixed and stained with anti-HRAS(A146T) monoclonal.