Anti-Rad23B (5H1) Mouse Monoclonal Antibody
Product Information
Downloads & Supports
Target
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RAD23B
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
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5H1-A10-A7
Isotype
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IgG2b
Reactivity
Application
Application Details
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WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Immunogen
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Purified recombinant human hHR23b protein fragments expressed in E.coli.
Molecular Weight
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Calculated MW: 43 kDa; Observed MW: 58 kDa
Formulation
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Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Form
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Liquid
Gene ID
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5887
Uniprot ID
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P54727
Background
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The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Research Area
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Epigenetics and Nuclear Signaling
Synonyms
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RAD23B; UV excision repair protein RAD23 homolog B; HR23B; hHR23B; XP-C repair-complementing complex 58 kDa protein; p58