Anti-PRAS40 Rabbit Monoclonal Antibody
Product Information
Downloads & Supports
Target
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AKT1S1
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
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R06-8A6
Isotype
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IgG
Reactivity
Application
Application Details
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WB: 1/500-1/1000 IP: 1/50
Immunogen
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A synthesized peptide derived from human PRAS40
Molecular Weight
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Calculated MW: 27 kDa; Observed MW: 40 kDa
Formulation
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Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
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Liquid
Gene ID
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84335
Uniprot ID
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Q96B36
Background
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PRAS40 interacts with Raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1. mTORC1 in turn phosphorylates PRAS40 at Ser183.
Research Area
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Cell Biology
Synonyms
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AKT1S1; PRAS40; Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate