Anti-PMS2 Rabbit Monoclonal Antibody
Product Information
Downloads & Supports
Target
-
PMS2
Host
Storage
-
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
-
R06-5G3
Isotype
-
IgG
Reactivity
Application
Application Details
-
WB: 1/500-1/1000 IP: 1/20
Immunogen
-
A synthetic peptide of human PMS2
Molecular Weight
-
Calculated MW: 96 kDa; Observed MW: 96 kDa
Formulation
-
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Form
-
Liquid
Gene ID
-
5395
Uniprot ID
-
P54278
Background
-
Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected.
Research Area
-
Epigenetics and Nuclear Signaling
Synonyms
-
DNA mismatch repair gene; DNA mismatch repair protein PMS2; HNPCC4; PMS1 protein homolog 2