Anti-PIK3CA(E542Q Mutant) Mouse Monoclonal Antibody

Anti-PIK3CA(E542Q Mutant) Mouse Monoclonal Antibody
Anti-PIK3CA(E542Q Mutant) Mouse Monoclonal Antibody
Anti-PIK3CA(E542Q Mutant) Mouse Monoclonal Antibody
Anti-PIK3CA(E542Q Mutant) Mouse Monoclonal Antibody

Anti-PIK3CA(E542Q Mutant) Mouse Monoclonal Antibody

Mouse IgG Monoclonal for PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha detection, Test with WB, IHC-P, ELISA in Vertebrates

Reactivity
  • Vertebrates
Application
  • WB
  • IHC-P
  • ELISA
Host
  • Mouse
Catalog Number
    ABXB07369
$680.00

Shipping To:
United States

Product Information

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Target
    PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha
Host
  • Mouse
Storage
    Store at -20°C. Avoid repeated freezing and thawing
Clonality
  • Monoclonal
Clone Number
    7E8-V5-R2
Isotype
    IgG
Reactivity
  • Vertebrates
Application
  • WB
  • IHC-P
  • ELISA
Application Details
    ELISA: 1/1000-1/5000 WB: 1/500-1/1000 IHC-P: 1/50-1/100
Immunogen
    A synthetic peptide from the internal region of PIK3CA which includes the mutation of E542Q, human origin.
Formulation
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Form
    Liquid
Uniprot ID
    ­
Background
    Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer.
Research Area
    Signal Transduction
Synonyms
    PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha
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Product Image

Western blot analysis of recombinant PIK3CA(E542Q) and wild type proteins. Purified His-tagged PIK3CA(E542Q) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-PIK3CA(E542Q) monoclonal.
Immunofluorescence of cells expressing PIK3CA proteins with Anti-PIK3CA(E542Q). HEK293T cells were transfected with pCDNA3-GFP-PIK3CA (WT) plasmid (left column) or pCDNA3-GFP-PIK3CA(E542Q) plasmid (right column), then fixed and stained with Anti-PIK3CA(E542Q) monoclonal.