Anti-Phospho-Src (Tyr530) Rabbit Monoclonal Antibody
Product Information
Downloads & Supports
Target
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SRC
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
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R03-8H8
Isotype
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IgG
Reactivity
Application
Application Details
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WB: 1/500-1/1000 IP: 1/20
Immunogen
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A synthetic phosphopeptide corresponding to residues surrounding Tyr529 of human Src
Molecular Weight
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Calculated MW: 60 kDa; Observed MW: 60 kDa
Formulation
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50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Form
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Liquid
Gene ID
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6714
Uniprot ID
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P12931
Background
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Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).
Research Area
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Signal Transduction
Synonyms
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SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src