Anti-Phospho-Smad3 (Ser423/425) Rabbit Polyclonal Antibody

Anti-Phospho-Smad3 (Ser423/425) Rabbit Polyclonal Antibody
Anti-Phospho-Smad3 (Ser423/425) Rabbit Polyclonal Antibody
Anti-Phospho-Smad3 (Ser423/425) Rabbit Polyclonal Antibody
Anti-Phospho-Smad3 (Ser423/425) Rabbit Polyclonal Antibody
Anti-Phospho-Smad3 (Ser423/425) Rabbit Polyclonal Antibody
Anti-Phospho-Smad3 (Ser423/425) Rabbit Polyclonal Antibody

Anti-Phospho-Smad3 (Ser423/425) Rabbit Polyclonal Antibody

Rabbit IgG Polyclonal for SMAD3 detection, Test with WB, IHC-P, ICC/IF in Human, Mouse

Reactivity
  • Human
  • Mouse
Application
  • WB
  • IHC-P
  • ICC
  • IF
Host
  • Rabbit
Catalog Number
    ABXB06453
$370.00

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United States

Product Information

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Target
    SMAD3
Host
  • Rabbit
Storage
    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
  • Polyclonal
Clone Number
    -
Isotype
    IgG
Reactivity
  • Human
  • Mouse
Application
  • WB
  • IHC-P
  • ICC
  • IF
Application Details
    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Immunogen
    A synthesized peptide derived from human Phospho-Smad3 (S423 + S425)
Molecular Weight
    Calculated MW: 48 kDa; Observed MW: 55 kDa
Formulation
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
    Liquid
Gene ID
    4088
Uniprot ID
    P84022
Background
    Smad3 transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.
Research Area
    Signal Transduction
Synonyms
    SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
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Western blot analysis of Phospho-Smad3(S423/S425) in PC-12 lysates treated with 1%BSA using Phospho-Smad3 (Ser423/425).
Immunohistochemistry analysis of paraffin-embedded Human gastric adenocarcinoma using Phospho-Smad3 (S423 + S425).High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Immunofluorescence analysis of Phospho-Smad3 (Ser423/425) in A549 treated with TGFβ, using Phospho-Smad3 (S423 + S425).