Anti-Phospho-Rb (Ser608) Rabbit Monoclonal Antibody
Product Information
Downloads & Supports
Target
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RB1
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
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R02-6C5
Isotype
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IgG
Reactivity
Application
Application Details
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WB: 1/500-1/1000 IHC: 1/50-1/100
Immunogen
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A synthetic phosphopeptide corresponding to residues surrounding Ser608 of human Rb
Molecular Weight
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Calculated MW: 106 kDa; Observed MW: 110 kDa
Formulation
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50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Form
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Liquid
Gene ID
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5925
Uniprot ID
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P06400
Background
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Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.
Research Area
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Cell Biology
Synonyms
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RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110