Anti-Phospho-Raf1 (Ser259) Rabbit Monoclonal Antibody
Product Information
Downloads & Supports
Target
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RAF1
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
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R07-4J4
Isotype
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IgG
Reactivity
Application
Application Details
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WB: 1/500-1/1000 IHC: 1/50-1/100
Immunogen
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A synthetic phosphopeptide corresponding to residues surrounding Ser259 of human Raf1
Molecular Weight
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Calculated MW: 73 kDa; Observed MW: 73 kDa
Formulation
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50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Form
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Liquid
Gene ID
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5894
Uniprot ID
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P04049
Background
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Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2.
Research Area
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Signal Transduction
Synonyms
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RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1