Anti-Phospho-NF-KB p65 (Thr254) Rabbit Polyclonal Antibody

Anti-Phospho-NF-KB p65 (Thr254) Rabbit Polyclonal Antibody
Anti-Phospho-NF-KB p65 (Thr254) Rabbit Polyclonal Antibody
Anti-Phospho-NF-KB p65 (Thr254) Rabbit Polyclonal Antibody
Anti-Phospho-NF-KB p65 (Thr254) Rabbit Polyclonal Antibody
Anti-Phospho-NF-KB p65 (Thr254) Rabbit Polyclonal Antibody
Anti-Phospho-NF-KB p65 (Thr254) Rabbit Polyclonal Antibody
Anti-Phospho-NF-KB p65 (Thr254) Rabbit Polyclonal Antibody
Anti-Phospho-NF-KB p65 (Thr254) Rabbit Polyclonal Antibody

Anti-Phospho-NF-KB p65 (Thr254) Rabbit Polyclonal Antibody

Rabbit IgG Polyclonal for RELA detection, Test with WB, IHC-P, ICC/IF in Human, Mouse, Rat

Reactivity
  • Human
  • Mouse
  • Rat
Application
  • WB
  • IHC-P
  • ICC
  • IF
Host
  • Rabbit
Catalog Number
    ABXB06499
$370.00

Shipping To:
United States

Product Information

Downloads & Supports

Target
    RELA
Host
  • Rabbit
Storage
    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
  • Polyclonal
Clone Number
    -
Isotype
    IgG
Reactivity
  • Human
  • Mouse
  • Rat
Application
  • WB
  • IHC-P
  • ICC
  • IF
Application Details
    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Immunogen
    Peptide sequence around phosphorylation site of threonine 254 (F-R-T(p)-P-P) derived from Human NFκB-p65.
Molecular Weight
    Calculated MW: 60 kDa; Observed MW: 65 kDa
Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Form
    Liquid
Gene ID
    5970
Uniprot ID
    Q04206
Background
    NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm.
Research Area
    Cell Biology
Synonyms
    NFKB3; RELA; TF65; Transcription factor p65; p65; NFkB
MSDSProtocolsRequest COAAlgent Product GuaranteeNeed support?

Product Image

Western blot analysis of Phospho-NF-KB p65 (Thr254) in 3T3 lysates untreated or treated with TNFα using NFκBp65 (Phospho-Thr254).
Immunofluorescence analysis of Phospho-NF-KB p65 (Thr254) in Hela using NFκBp65(Phospho-Thr254).
Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue using NFκBp65 (Phospho-Thr254) (left) or the same preincubated with blocking peptide (right).High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Western blot analysis of Phospho-NF-KB p65 (Thr254) in 3T3 lysates untreated or treated with Alkaline phosphatase using Phospho-NF-KB p65 (Thr254)(upper)or βActin(2000688F10, lower).