Anti-Phospho-HSF1 (Ser326) Rabbit Monoclonal Antibody
Product Information
Downloads & Supports
Target
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HSF1
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
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R77-7H-8
Isotype
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IgG
Reactivity
Application
Application Details
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WB: 1/500-1/1000 IHC: 1/100-1/200 IF: 1/50-1/200 IP: 1/50 FC: 1/50
Immunogen
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A synthesized peptide derived from human Phospho-HSF1 (S326)
Molecular Weight
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Calculated MW: 57 kDa;Observed MW:82kDa
Formulation
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Liquid in PBS , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
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Liquid
Gene ID
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3297
Uniprot ID
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Q00613
Background
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Heat shock transcription factors (HSF, also designated HSTF) 1 and 2 are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development, and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway.
Research Area
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Tags & Cell Markers
Synonyms
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HSF1; HSTF 1; HSTF1; Heat shock factor protein 1; Heat shock transcription factor 1;