Anti-Phospho-eIF4E (Ser209) Rabbit Monoclonal Antibody
Product Information
Downloads & Supports
Target
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EIF4E
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
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R77-8A-2
Isotype
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IgG
Reactivity
Application
Application Details
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WB: 1/500-1/1000 IHC: 1/100-1/200 IF: 1/50-1/200 IP: 1/50
Immunogen
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A synthesized peptide derived from human Phospho-eIF4E (S209)
Molecular Weight
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Calculated MW: 25 kDa;Observed MW:25kDa
Formulation
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Liquid in PBS , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
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Liquid
Gene ID
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1977
Uniprot ID
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P06730
Background
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eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.
Research Area
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Epigenetics and Nuclear Signaling
Synonyms
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CBP; eIF-4E; eIF-4F 25 kDa subunit; EIF4F; EIF4E1; EIF4EL1; MGC111573; EIF4E;