Anti-Phospho-CRK (Tyr221) Rabbit Monoclonal Antibody
Product Information
Downloads & Supports
Target
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CRK
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
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R04-6L8
Isotype
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IgG
Reactivity
Application
Application Details
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WB: 1/500-1/1000 IF: 1/50-1/200
Immunogen
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A synthesized peptide derived from human Phospho-CrkII (Tyr221)
Molecular Weight
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Calculated MW: 34 kDa; Observed MW: 42 kDa
Formulation
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Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
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Liquid
Gene ID
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1398
Uniprot ID
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P46108
Background
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The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.
Research Area
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Cell Biology
Synonyms
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Adapter molecule crk; avian sarcoma virus CT10 (v-crk) oncogene homolog; CRK; CRKII; Proto-oncogene c-Crk