Anti-Nucleophosmin (2D9) Mouse Monoclonal Antibody
Product Information
Downloads & Supports
Target
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NPM1
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
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2D9-C3-E5
Isotype
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IgG1
Reactivity
Application
Application Details
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WB: 1/500-1/1000 ChIP: 1/20
Immunogen
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Purified recombinant full length of human NPM1 protein expressed in E.coli.
Molecular Weight
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Calculated MW: 33 kDa; Observed MW: 38 kDa
Formulation
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Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Form
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Liquid
Gene ID
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4869
Uniprot ID
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P06748
Background
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Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029).
Research Area
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Epigenetics and Nuclear Signaling
Synonyms
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B23; NPM