Anti-NCE2 Rabbit Monoclonal Antibody
Product Information
Downloads & Supports
Target
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UBE2F
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
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R05-8C4
Isotype
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IgG
Reactivity
Application
Application Details
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WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Immunogen
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Recombinant protein of human NCE2
Molecular Weight
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Calculated MW: 21 kDa; Observed MW: 21 kDa
Formulation
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50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Form
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Liquid
Gene ID
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140739
Uniprot ID
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Q969M7
Background
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Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
Research Area
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Epigenetics and Nuclear Signaling
Synonyms
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NCE2; NEDD8 carrier protein UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2