Anti-MEK1 Rabbit Monoclonal Antibody
Product Information
Downloads & Supports
Target
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MAP2K1
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
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R09-5B-9
Isotype
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IgG
Reactivity
Application
Application Details
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WB：1/500-1/1000
Immunogen
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Peptide
Molecular Weight
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Calculated MW: 43 kDa; Observed MW:43 kDa
Formulation
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Liquid in PBS, Glycerol and BSA
Form
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Liquid
Gene ID
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5604
Uniprot ID
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Q02750
Background
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Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation.
Research Area
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Cell Biology
Synonyms
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MAP2K1, MEK1, PRKMK1