Anti-M-RIP Rabbit Polyclonal Antibody
Product Information
Downloads & Supports
Target
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MPRIP
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clonality
Clone Number
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-
Isotype
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IgG
Reactivity
Application
Application Details
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WB: 1/500-1/1000 IHC: 1/50-1/200 IP: 1/50-1/100
Immunogen
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Recombinant protein of human M-RIP.
Molecular Weight
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Calculated MW: 117kDa
Formulation
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1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Form
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Liquid
Gene ID
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23164
Uniprot ID
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Q6WCQ1
Background
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Myosin phosphatase-rho interacting protein (M-RIP), also known as p116RIP, RIP3, and MPRIP, localizes to actin-myosin filaments regulating cytoskeletal dynamics. M-RIP contains amino-terminal pleckstrin homology domains, carboxyl-terminal coiled-coil domains, and was originally identified to associate with the myosin phosphatase complex. M-RIP binds to MBS/MYRT, the myosin binding subunit of myosin phosphatase, as well as RhoA. Phosphorylation of MYRT by Rho-associated kinase (ROCK) inhibits myosin phosphatase activity, resulting in increased levels of phosphorylation on myosin light chain, and enhanced contractility. M-RIP may function as a scaffolding protein for the complex between the myosin phosphatase complex, Rho/ROCK, and actin. Silencing of M-RIP results in disassembly of the complex, increased phosphorylation of myosin light chain, and changes to cytoskeletal dynamics.
Research Area
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Synonyms
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Myosin phosphatase Rho-interacting protein;
M-RIP;Rho-interacting protein 3;RIP3;p116Rip;MPRIP