Anti-IDH2(R140Q Mutant) Mouse Monoclonal Antibody

Anti-IDH2(R140Q Mutant) Mouse Monoclonal Antibody
Anti-IDH2(R140Q Mutant) Mouse Monoclonal Antibody
Anti-IDH2(R140Q Mutant) Mouse Monoclonal Antibody
Anti-IDH2(R140Q Mutant) Mouse Monoclonal Antibody

Anti-IDH2(R140Q Mutant) Mouse Monoclonal Antibody

Mouse IgG Monoclonal for IDH2, D2HGA2, ICD-M, IDH, IDHM, IDP, IDPM, mNADP-IDH detection, Test with WB, IHC-P, ICC/IF, ELISA in Vertebrates

Reactivity
  • Vertebrates
Application
  • WB
  • IHC-P
  • ICC
  • IF
  • ELISA
Host
  • Mouse
Catalog Number
    ABXB07339
$680.00

Shipping To:
United States

Product Information

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Target
    IDH2, D2HGA2, ICD-M, IDH, IDHM, IDP, IDPM, mNADP-IDH
Host
  • Mouse
Storage
    Store at -20°C. Avoid repeated freezing and thawing
Clonality
  • Monoclonal
Clone Number
    4Q7-A3-T1
Isotype
    IgG
Reactivity
  • Vertebrates
Application
  • WB
  • IHC-P
  • ICC
  • IF
  • ELISA
Application Details
    ELISA: 1/1000-1/5000,WB: 1/500-1/1000,ICC/IF: 1/50-1/100,IHC-P: 1/50-1/100
Immunogen
    A synthetic peptide from the internal region of IDH2 which includes the mutation of R140Q, human origin.
Formulation
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Form
    Liquid
Uniprot ID
    ­
Background
    Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.
Research Area
    Signal Transduction
Synonyms
    IDH2, D2HGA2, ICD-M, IDH, IDHM, IDP, IDPM, mNADP-IDH
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Product Image

Western blot analysis of recombinant IDH2(R140Q) and wild type proteins.Purified His-tagged IDH2(R140Q) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with anti IDH2(R140Q) monoclonal.
Immunofluorescence of cells expressing IDH2 proteins with anti IDH2(R140Q).HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2(R140Q) plasmid (right column), then fixed and stained with anti-IDH2(R140Q) monoclonal.