Anti-IDH1(R132P Mutant) Mouse Monoclonal Antibody
Product Information
Downloads & Supports
Target
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IDH2, D2HGA2, ICD-M, IDH, IDHM, IDP, IDPM, mNADP-IDH
Host
Storage
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Store at -20°C. Avoid repeated freezing and thawing
Clonality
Clone Number
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5Z9-W9-O8
Isotype
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IgG
Reactivity
Application
Application Details
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ELISA: 1/1000-1/5000 WB: 1/100-1/1000 ICC/IF: 1/50-1/100 IHC-P: 1/50-1/100
Immunogen
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A synthetic peptide from the internal region of IDH1 which includes the mutation of R132P, human origin.
Formulation
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PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Form
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Liquid
Uniprot ID
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Background
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Isocitrate dehydrogenases (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.
Research Area
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Signal Transduction
Synonyms
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IDH2, D2HGA2, ICD-M, IDH, IDHM, IDP, IDPM, mNADP-IDH